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pTA-M+

Plasmid

#249818

Purpose

Expresses Mycobacterium tuberculosis topoisomerase I under the control of the Tet promoter in mycobacteria

Depositor

Yuk-Ching Tse-Dinh

Article

Sandhaus et al Antimicrob Agents Chemother. 2016 Jun 20

Insert

Mycobacterium tuberculosis topoisomerase I (topA Mycobacterium tuberculosis)

Tags

6xHis

Expression

Bacterial

Promoter

Tet

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmBorealin-OLLAS

Plasmid

#237439

Purpose

Expresses mouse Borealin tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

Insert

Borealin (Cdca8 Mouse)

Tags

OLLAS

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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Bm iPGM-HiBiT

Plasmid

#218483

Purpose

Bacterially expressed iPGM-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Bm-iPGM-C-HiBIT-Thrombin-6XHis

Tags

HiBiT Tag and His-tag with thrombin site

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLAS2w.Phyg-CHST8

Plasmid

#232650

Purpose

expresses CHST8 wild-type in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLAS2w.Phyg-CHST8 (3A)

Plasmid

#232651

Purpose

expresses CHST8 REP-to-AAA mutant in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (3A) (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R258A, E259A, P260A

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc RBM42 deletion

Plasmid

#229503

Purpose

Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_Del 363

Expression

Mammalian

Mutation

Deletion of bp 363-394

Promoter

SV40

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc Compensatory Mutant

Plasmid

#229504

Purpose

Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_CompMutant

Expression

Mammalian

Mutation

Mutations in bp 363-394

Promoter

SV40

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-455-WT

Plasmid

#215881

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-I-type

Plasmid

#215883

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(5p)-I-type

Plasmid

#215884

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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