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We narrowed to 11,390 results for: mpl

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Showing: 11371 - 11390 of 11390 results

  3. cAMP-dependent protein kinase (PKA) regulatory 1a subunit (R1a)

    Plasmid
    #215436
    Purpose
    Bacterially expressed R1a (no HiBiT) Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human cAMP-dependent protein kinase type I-alpha subunit (PRKAR1A) mRNA, complete cds, C-terminal 10X-His.Thrombin Tag
    Tags
    10X His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215436-plasmid-map-sequence-id-428696

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  4. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

    Plasmid
    #194608
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Deletion of N-terminal CARD domain and changed Cy…
    Promoter
    CMV
    Available Since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194608-plasmid-map-sequence-id-384891

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  5. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS

    Plasmid
    #194926
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 325 to Alanine to Eliminate Prot…
    Promoter
    CMV
    Available Since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194926-plasmid-map-sequence-id-384890

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  6. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192704-plasmid-map-sequence-id-379659

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  12. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔLD + ΔSS

    Plasmid
    #194610
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Linker + Small Subunit Domain
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Ala 354 to Ser 454 (Casp9 Mouse)
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 325 to Alanine to eliminate prot…
    Promoter
    CMV
    Available Since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194610-plasmid-map-sequence-id-384887

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  13. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

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  14. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  15. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Available Since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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  18. pJTB126

    Plasmid
    #248910
    Purpose
    expresses the yeast analog-sensitive Smk1-Q120A and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Rimal et al Mol Cell Biol. 2020 Jul 29;40(16). pii:
    Inserts
    SMK1 Mitogen-activated Protein Kinase -analog-sensitive form (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes from S288C form, Budding Yeast)
    Ssp2 Activator of the Smk1 MAPK (SSP2 Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form, Budding Yeast)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    codon 120 has been changed from Q to A (Smk1-Q120…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248910-plasmid-map-sequence-id-497716

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  19. pDONR201-JIP60ml

    Plasmid
    #104959
    Purpose
    Encodes a modified, active, form of Hordeum vulgare c.v. Golden Promise Jasmonate Induced Protein 60 (JIP60)
    Depositor
    Pietro Spanu
    Article
    Pennington et et al Figshare (2018)
    Insert
    Jasmonate Induced Protein 60 (JIP60)
    Use
    Gateway entry vector for lr recombination into ot…
    Mutation
    Replaced amino acids 164 to 187 with a methionine…
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104959-plasmid-map-sequence-id-204404

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  20. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72427-plasmid-map-sequence-id-137744

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Showing: 11371 - 11390 of 11390 results