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EBFP2-CytERM-N-17

Plasmid

#55238

Purpose

Localization: Endoplasmic Reticulum, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

CytERM

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hSyn-GRAB-rACh1h

Plasmid

#234412

Purpose

Expresses the red ACh sensor GRAB rACh1h in neurons

Depositor

Yulong Li

Publication

Xie et al Nat Neurosci. 2026 Jun 16. doi: 10.1038/

Insert

GRAB-rACh1h

Use

AAV

Promoter

hSyn

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pET22b-msfGFP-NPM1

Plasmid

#237670

Purpose

For expression of msfGFP-NPM1 in Ecoli

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

msfGFP

Expression

Bacterial

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Publication

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC081: pAAV.CMV.mCherry

Plasmid

#205744

Purpose

AAV vector for expressing CMV-driven mCherry reporter gene.

Depositor

Guei-Sheung Liu

Publication

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LEU (V5 and HA)

Plasmid

#222486

Purpose

Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Publication

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pYDE007

Plasmid

#194152

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Publication

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a

Plasmid

#183567

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)

Depositor

Erik Dent

Publication

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAM12

Plasmid

#86471

Purpose

Expresses p12 and p9-mCitrine

Depositor

Pamela Silver

Publication

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9-mCitrine

Use

Synthetic Biology

Tags

mCitrine

Expression

Bacterial

Promoter

pBAD

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSHDY-PL03-mVenus_tetR

Plasmid

#137663

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.

Depositor

Ilka Axmann

Publication

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

PL03:mVenus

Expression

Bacterial

Promoter

PL03

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-FKBP

Plasmid

#128771

Purpose

Mammalian expression

Depositor

Colin Taylor

Publication

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FKBP

Expression

Mammalian

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1306

Plasmid

#111128

Purpose

Expresses FANCE.

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCE (FANCE Human)

Use

Lentiviral

Tags

HA

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHJ2: LexA DBD

Plasmid

#108240

Purpose

LexA DNA binding domain

Depositor

Jerome Bonnet

Publication

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain (1-87)

Use

Synthetic Biology

Promoter

pLacO1

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGLACTE-HT51-10

Plasmid

#99255

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.

Depositor

Karen Usdin

Publication

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Human)

Tags

eGFP

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pJL101

Plasmid

#243685

Purpose

Stably expresses LOV-BirA-GFP-OMP25 TMD fusion proteins, localized to mitochondrial outer membrane

Depositor

Jonathan Weissman

Publication

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

LOV-BirA-GFP-OMP25

Use

Lentiviral

Promoter

EF1a

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-control

Plasmid

#238257

Purpose

For overexpression of EGFP-control

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pNDL-54

Plasmid

#161715

Purpose

In vitro transcription construct for production of membrane acvr1b-Cry2 mRNA

Depositor

Adam Cohen

Publication

McNamara et al Development. 2025 May 1;152(9):dev204506

Insert

Myr-acvr1b-Cry2 (acvr1ba Zebrafish)

Use

In vitro transcription

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 1

Plasmid

#206992

Purpose

Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Publication

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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