We narrowed to 29,768 results for: REP

1. pET24a_UDP_Xase_E113

   Plasmid

   #73159

   Purpose

   Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

   Depositor

   Sandra Gabelli

   Article

   Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

   Insert

   pET24a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   change glutamate 113 to alanine

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pMCh-139

   Plasmid

   #92287

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

   Insert

   dGW182 NED (gw Fly)
   

   Use

   Tags

   NHA

   Expression

   Insect

   Mutation

   dGW182 NED: aa 205-490

   Promoter

   Ac5

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Luc-Nanog-3'UTRmut

   Plasmid

   #63894

   Purpose

   Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

   Depositor

   Lin He

   Article

   Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

   Insert

   Nanog homeobox (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-mETV3-HA

   Plasmid

   #33375

   Depositor

   Peter Murray

   Article

   El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

   Insert

   Etv3 (Etv3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pFP121

   Plasmid

   #21670

   Depositor

   James Haber

   Article

   Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

   Insert

   Lacz (lacZ E. coli)
   

   Use

   Centromeric plasmid

   Tags

   Expression

   Mutation

   The second lacZ sequence has been modified, the E…

   Promoter

   Available since

   Sept. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-hfYFP-TEV-SAV

   Plasmid

   #191197

   Purpose

   Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-LSSmGFP-TEV-SAV

   Plasmid

   #191200

   Purpose

   Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB65

   Plasmid

   #226319

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis- (VTG to AAA MR1-6)-strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRAR092-L31

    Plasmid

    #185045

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31 coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRAR112

    Plasmid

    #185048

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region K2A mutation, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K2A amino acid mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRAR113

    Plasmid

    #185049

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H6A amino acid mutaitons

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRAR114

    Plasmid

    #185050

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E8A amino acid mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRAR120

    Plasmid

    #185041

    Purpose

    J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Only first 90 nt of rpmE coding present

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRAR089-L36p

    Plasmid

    #185042

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L36p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAR090-L36

    Plasmid

    #185043

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L36
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRAR091-L31p

    Plasmid

    #185044

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTcI38-PRM-wasabi

    Plasmid

    #195554

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI38
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pS44i8GH-2

    Plasmid

    #207526

    Purpose

    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYUBcLIC-GFP

    Plasmid

    #183509

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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