We narrowed to 15,178 results for: gan

1. pET21-pelB-GST-8

   Plasmid

   #228990

   Purpose

   For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-8
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_YWHAZ_14-3-3

   Plasmid

   #109808

   Purpose

   Protein expression and purification of YWHAZ_14-3-3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   YWHAZ_14-3-3 (YWHAZ Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

   Plasmid

   #187076

   Purpose

   Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments

   Depositor

   Kai Johnsson

   Article

   Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

   Insert

   TOM20-dHaloTag7_T2A_Lifeact-HaloTag7
   

   Expression

   Mammalian

   Mutation

   dHaloTag7 = HaloTag7-D106A

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSERT pcDNA3

   Plasmid

   #15483

   Depositor

   Randy Blakely

   Article

   Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

   Insert

   hSERT (SLC6A4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra

   Plasmid

   #24129

   Purpose

   3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

   Depositor

   Malcolm Moore

   Article

   Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_RAD51_Rad51

   Plasmid

   #110031

   Purpose

   Protein expression and purification of RAD51_Rad51

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RAD51_Rad51 (RAD51 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_TetO_CD19-P2A-mCherry

   Plasmid

   #133803

   Purpose

   repressible expression of CD19 and mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   putative ECD of H. sapiens CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAS-V5-miniTurboID-NES

    Plasmid

    #116905

    Purpose

    Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurboID
    

    Use

    Drosophila expression

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-HOT_tdTomato

    Plasmid

    #138567

    Purpose

    Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    tdTomato
    

    Use

    Unspecified

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry N1 TOMM22

    Plasmid

    #157762

    Purpose

    Expression of TOMM22:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    TOMM22 (TOMM22 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-SPN_ARR wt (Shank3)

    Plasmid

    #175254

    Purpose

    SPN-ARR fragment in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet30b-5M SrtA

    Plasmid

    #51140

    Purpose

    expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Article

    Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Tags

    KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LPC-flag-REST-WT

    Plasmid

    #41903

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid

    #89347

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-RhoA

    Plasmid

    #23224

    Depositor

    Channing Der

    Article

    Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

    Insert

    RhoA (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only