We narrowed to 17,085 results for: Por

1. pHD-SPARC2-D-LexA::p65

   Plasmid

   #133562

   Purpose

   Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. OMM-long-CFAST10

   Plasmid

   #233598

   Purpose

   Expression of CFAST10 on the OMM membrane after a long linker

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   CFAST10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynapsin- soCoChR-GFP

   Plasmid

   #107708

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Has Service

   AAV9

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-RON-V5/HIS

   Plasmid

   #201983

   Purpose

   expression of human RON receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   RON receptor tyrosine kinase (MST1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LUC05 (L/Cys/P)

   Plasmid

   #112146

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-EF1A-FHA-Linker-eGFP

   Plasmid

   #176065

   Purpose

   EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   FHA
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLM-mCitrine-Sox2

   Plasmid

   #23242

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHD-SPARC2-I-LexA::p65

   Plasmid

   #133563

   Purpose

   Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

   Plasmid

   #194692

   Purpose

   hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HaloTag-WDR5

    Plasmid

    #200880

    Purpose

    Mammalian expression of human WDR5 with N-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HaloTag-WDR5 (WDR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid

    #207636

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SpyCatcher’s critical lysine

    Promoter

    CMV

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWPI-UPP1-3xFLAG

    Plasmid

    #201644

    Purpose

    Over-expression of 3xFLAG-tagged human UPP1

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-FKBP-GFP-NDP52

    Plasmid

    #135296

    Purpose

    stably express GFP-tagged NDP52 for CID induced mitophagy

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YCplac33-NAT_MCART1

    Plasmid

    #140591

    Purpose

    Express MCART1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon-optimized for expression in S. cerevisiae

    Promoter

    NDT1 promoter and 3'UTR

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-MCU-D261A

    Plasmid

    #31729

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Changed D261 to Alanine

    Promoter

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB80-LAMP1-mCherry-iLID

    Plasmid

    #174625

    Purpose

    Optogenetic coupling to lysosomes (LAMP1) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    LAMP1-mCherry-iLID (LAMP1 Synthetic, Human)
    

    Use

    Tags

    mCherry-iLID

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-Hygro-EF1A-Macro-Linker-eGFP

    Plasmid

    #176067

    Purpose

    EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    Macro
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-MCU-E264A

    Plasmid

    #31730

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Changed E264 to Alanine

    Promoter

    None

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1alpha-soCoChR-tdTomato

    Plasmid

    #107714

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-tdTomato
    

    Use

    AAV

    Tags

    KA2 and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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