We narrowed to 9,004 results for: ski

1. pDONR223_AKR1B1_p.F252L

   Plasmid

   #82743

   Purpose

   Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   AKR1B1 (AKR1B1 Human)
   

   Use

   Gateway entry vector

   Mutation

   F252L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_STC2_p.R5W

   Plasmid

   #82744

   Purpose

   Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   STC2 (STC2 Human)
   

   Use

   Gateway entry vector

   Mutation

   R5W

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBrain-GFP-TACC3KDP(S558A)-shTACC3

   Plasmid

   #59357

   Purpose

   Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRIB1 gRNA (BRDN0001162240)

   Plasmid

   #76661

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB1 (TRIB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIB1 gRNA (BRDN0001162480)

   Plasmid

   #76662

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB1 (TRIB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRIB1 gRNA (BRDN0001162575)

   Plasmid

   #76663

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB1 (TRIB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRIB1 gRNA (BRDN0001148054)

   Plasmid

   #76664

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB1 (TRIB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)

   Plasmid

   #52129

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   Jnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-470 and changed C478S, C667…

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)

   Plasmid

   #52130

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   Jnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-470 and changed C478S, C643…

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)

    Plasmid

    #52131

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    Jnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-470 and changed C478S, C631…

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)

    Plasmid

    #52133

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-470 and C478S, C631S, C643S,…

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

    Plasmid

    #52134

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-281 and C605S, C631S, C643S …

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pT3-EF1aH Yap S127A

    Plasmid

    #86497

    Purpose

    Mammalian expression of FLAG-tagged Yap containing S127A mutation

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes associated protein 1 (YAP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. COVID-SARS2 NSP14/10

    Plasmid

    #159613

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

    Depositor

    Opher Gileadi

    Article

    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

    Inserts

    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    

    Tags

    His6, TEV cleavage site and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-FFSS-Cyclin D1

    Plasmid

    #215151

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV2/9 REP-AAP-ΔCAP

    Plasmid

    #205991

    Purpose

    Rep and AAP protein expression for AAV CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    AAV9 Rep and AAP proteins for CREATE libraries
    

    Use

    AAV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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