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HA-FOXO3a TM

Plasmid

#1788

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A (no longer detectably phosphor…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-NICD1

Plasmid

#46047

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc tag

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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deltaNp63alpha-FLAG

Plasmid

#26979

Depositor

David Sidransky

Article

Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

Insert

ΔNP63alpha (TP63 Human)

Tags

FLAG

Expression

Mammalian

Mutation

dominant negative isoform of p63; aa 1–587

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgEGFP

Plasmid

#86153

Purpose

Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

EGFP

Use

CRISPR and Lentiviral

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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R26-H2B-EGFP HR donor vector

Plasmid

#137925

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Mutation

contains an altered Cre gene modified by the addi…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGW1-mCherry-EGFP-PIM

Plasmid

#111759

Purpose

High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

CMV

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PRB

Plasmid

#89130

Purpose

mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDOC-GG

Plasmid

#149377

Purpose

Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Inserts

Fragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7repeats)-GFP-Pho8

Plasmid

#207011

Purpose

Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

GFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB-wt

Plasmid

#58905

Purpose

mammalian expression of wild type human Rb

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLAMP1-emiRFP670

Plasmid

#136570

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LAMP1-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-tubulin

Plasmid

#136573

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670-tubulin

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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