We narrowed to 10,460 results for: iCre
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Plasmid#133799PurposeCMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Leu-AcrIIA1
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)
Plasmid#133800PurposeCMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Eriv-AcrIIA1
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW070
Plasmid#134138PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationresidues 1-131, CRISPR resistantAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 D353A
Plasmid#133016PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationD353APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 C351A
Plasmid#133014PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationC351APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsa-cdk2 3'UTR sicheck2
Plasmid#121125Purposeluciferase assayDepositorInserthsa-cdk2 3'UTR (CDK2 Human)
ExpressionBacterialAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-2xmiR-1 target sites
Plasmid#120299PurposeAAV vector for expression of AcrIIA4 with two miR-1 binding sitesDepositorInsertAcrIIA4-2xmiR-1 binding sites
UseAAV and CRISPRTagsFLAGExpressionMammalianAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)
Plasmid#120300PurposeAAV vector for expression of AcrIIC1 (no miR binding sites, control vector)DepositorInsertAcrIIC1
UseAAV and CRISPRExpressionMammalianAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
ExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hARPC2
Plasmid#112549Purpose3'UTR reporter assayDepositorInsertARPC2 (ARPC2 Human)
UseLuciferaseAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-T46C
Plasmid#110543PurposeBacterial expression of E.coli DHFR C85A/C152S/T46C mutantDepositorInsertecDHFR
ExpressionBacterialMutationT46 mutated to C46, C85 mutated to A85, C152 muta…PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-L54C
Plasmid#110542PurposeBacterial expression of E.coli DHFR C85A/C152S/L54C mutantDepositorInsertecDHFR
ExpressionBacterialMutationL54 mutated to C54, C85 mutated to A85, C152 muta…PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-PD-H166Q
Plasmid#110179PurposeExpresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)DepositorInsertPropanediol Dehydratase
TagsHis6 and S-tagExpressionBacterialMutationH166Q mutantPromoterT7Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-PD-E440Q
Plasmid#110178PurposeExpresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3)DepositorInsertPropanediol Dehydratase
TagsHis6 and S-tagExpressionBacterialMutationE440Q mutantPromoterT7Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-PD-C438S
Plasmid#110177PurposeExpresses propanediol dehydratase mutant C438S in E. coli BL21(DE3)DepositorInsertPropanediol Dehydratase
TagsHis6 and S-tagExpressionBacterialMutationC438S mutantPromoterT7Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-PD-G817A
Plasmid#110174PurposeExpresses propanediol dehydratase mutant G817A in E. coli BL21(DE3)DepositorInsertPropanediol Dehydratase
TagsHis6 and S-tagExpressionBacterialMutationG817A mutantPromoterT7Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
SP65U1-135a-1
Plasmid#105819Purposeplasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmidDepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflCT-o4
Plasmid#101313PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o3
Plasmid#101311PurposeContains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only