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pMpGWB102

Plasmid

#68556

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pEG545

Plasmid

#40116

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Type

Empty backbone

Use

Gateway Cloning

Tags

Dendra2 PAFP (sequence optimized for worm express…

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pISH-beta2-microglobulin

Plasmid

#15883

Depositor

Peter Mombaerts

Article

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

beta2-microglobulin (B2m Mouse)

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
dCas9-CBP FL

Plasmid

#179550

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-TBK1-C-FLAG-HA

Plasmid

#131792

Purpose

Lentiviral vector expressing WT TBK1 containing STOP codon

Depositor

Alicia Pickrell

Article

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

STOP codon

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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TGFbetaR1 (T204D)-pcw107-V5

Plasmid

#64629

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TGFBR1 (transcript variant 1) (TGFBR1 Human)

Use

Lentiviral

Tags

V5

Mutation

T204D

Promoter

PGK

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pTK91_GFP-MRLC2

Plasmid

#46355

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

MRLC2 (MYL12B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-BSD

Plasmid

#174819

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-BSD

Tags

P2A-BSD and SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PIA900

Plasmid

#104401

Purpose

Polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA, B, C, and Z

Use

E. coli expression vector

Tags

encodes a C-terminally His-tagged rpoC which can …

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTK82_GFP-4.1G

Plasmid

#46353

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-LacZ

Plasmid

#18816

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-LacZ

Expression

Mammalian

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-RapR-FAK-YM

Plasmid

#25928

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse, Human)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag RET

Plasmid

#20614

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RET (RET Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

R982C

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR R4-vas2-integrase-R3

Plasmid

#62299

Purpose

phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

phageC31 integrase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pENO1-BFP-NAT

Plasmid

#50046

Purpose

Expression of BFP in Candida albicans

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

BFP

Expression

Yeast

Promoter

pENO1

Available Since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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