We narrowed to 15,934 results for: jun
-
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Synthetic, Mouse)
Tags3xFLAG, Avi, and TEVExpressionMammalianPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v2
Plasmid#173786PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 2DepositorInsertSpike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v3
Plasmid#173787PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 3DepositorInsertSpike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v6
Plasmid#173791PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 6DepositorInsertSpike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-deltaH69/V70
Plasmid#171744PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variantDepositorInsertSpike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV137
Plasmid#124256PurposeLentiviral vector (pRRL) containing Her2-ITD specific T cell receptorDepositorAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Tail-8A8G
Plasmid#157981PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tail-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPMutationmutant KRAS4B-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)
Plasmid#156405PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codonDepositorAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-Esrrb gRNA
Plasmid#128841PurposegRNA for targeting mouse Esrrb locus using CRISPR-cas techniqueDepositorInsertEsrrb gRNA (Esrrb Mouse)
UseCRISPRAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-ACSL3-sfGFP(C) HDR template
Plasmid#129413PurposeHDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFPDepositorInsertACSL3 HDR template (ACSL3 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP5
Plasmid#128939PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, ZDepositorInsertsTagsHisExpressionBacterialMutationResidues 234-241 are substituted for a PreScissio…PromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIE-C-hα2M-H6
Plasmid#128494PurposeExpresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.DepositorInsertA2M (R787-N1473) (A2M Human)
TagsHis tag (6x)ExpressionInsectMutationcontains R787-N1473PromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-26a-5p
Plasmid#103379PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-26a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-26a-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-5p
Plasmid#103392PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-5p target (MIR29A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LSB-hsa-miR-96-5p
Plasmid#103761PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-96-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-9-5p
Plasmid#103749PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-9-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-9-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only