We narrowed to 14,951 results for: pET

1. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-PDZD8HA_sh_resistant

   Plasmid

   #105006

   Purpose

   Mammalian Expression of PDZD8, sh resistant

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   PDZD8 (Pdzd8 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The sequence recognized by shPDZD8 (TRCN000043105…

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-mCherry-LacR-PALB2

   Plasmid

   #71112

   Purpose

   mammalian expession of mCherry-LacR-PALB2

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAP003l2

   Plasmid

   #209748

   Purpose

   RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

   Depositor

   Ahmed Badran

   Article

   Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

   Insert

   NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-3xFlag-ETO

   Plasmid

   #12507

   Depositor

   Dong-Er Zhang

   Article

   Biggs et al Mol Cell Biol. 2006 Oct . 26(20):7420-9.

   Insert

   ETO (RUNX1T1 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-iCre

   Plasmid

   #125577

   Purpose

   Expresses iCre under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   iCre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1 RagA shRNA #1

   Plasmid

   #30319

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagA shRNA (RRAGA Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pGEM-sgMUC1_dual-MTS-3x-Set I

   Plasmid

   #162763

   Purpose

   Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pK-myc-Par6C

   Plasmid

   #15474

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Insert

   Partitioning-defective protein 6C (PARD6A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

10. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO.1 RagA shRNA #2

    Plasmid

    #30320

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagA shRNA (RRAGA Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

13. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Marathon-RT tetramutant for Split-PE (pEH101)

    Plasmid

    #190109

    Purpose

    Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW-GFP-CtIP-T847E

    Plasmid

    #71111

    Purpose

    mammalian expession of GFP-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. MUNC18-2

    Plasmid

    #186687

    Purpose

    Expresses MUNC18-2 in insect cells

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    MUNC18-2 (Stxbp2 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag pLJM1 RagD 77L

    Plasmid

    #19317

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding D (RRAGD Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S77L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. plc242-GFP-Nprl2

    Plasmid

    #87051

    Purpose

    mammalian expression of Nprl2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge