We narrowed to 9,774 results for: pho

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pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TAL1(11))-PGKpuro2ABFP-W

Plasmid

#208545

Purpose

Lentiviral vector expressing gRNA targeting human TAL1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

TAL1(11) (TAL1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TAL1(12))-PGKpuro2ABFP-W

Plasmid

#208546

Purpose

Lentiviral vector expressing gRNA targeting human TAL1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

TAL1(12) (TAL1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(MRPS26(15))-PGKpuro2ABFP-W

Plasmid

#200499

Purpose

Lentiviral vector expressing gRNA targeting human MRPS26

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

MRPS26(15) (MRPS26 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#200502

Purpose

Lentiviral vector expressing gRNA targeting human AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(MRPS26(12))-PGKpuro2ABFP-W

Plasmid

#200498

Purpose

Lentiviral vector expressing gRNA targeting human MRPS26

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

MRPS26(12) (MRPS26 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NDUFA1(12))-PGKpuro2ABFP-W

Plasmid

#200495

Purpose

Lentiviral vector expressing gRNA targeting human NDUFA1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

NDUFA1(12) (NDUFA1 Human)

Use

Lentiviral

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(MRPL21(16))-PGKpuro2ABFP-W

Plasmid

#200497

Purpose

Lentiviral vector expressing gRNA targeting human MRPL21

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

MRPL21(16) (MRPL21 Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NDUFA1(11))-PGKpuro2ABFP-W

Plasmid

#200494

Purpose

Lentiviral vector expressing gRNA targeting human NDUFA1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

NDUFA1(11) (NDUFA1 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(MRPL21(14))-PGKpuro2ABFP-W

Plasmid

#200496

Purpose

Lentiviral vector expressing gRNA targeting human MRPL21

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

MRPL21(14) (MRPL21 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA K230A

Plasmid

#210811

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L2_sgRNA1

Plasmid

#201602

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L2 (LUC7L2 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Zebrafish IntS6

Plasmid

#198410

Purpose

Expresses FLAG-tagged zebrafish IntS6

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

ints6 (ints6 Zebrafish)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Zebrafish IntS6L

Plasmid

#198411

Purpose

Expresses FLAG-tagged zebrafish IntS6L

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

ints6l (ints6l Zebrafish)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Hy_U5:34A eGFP SV40

Plasmid

#195064

Purpose

Expresses eGFP when there is readthrough transcription from the Drosophila U5:34A gene

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

snRNA:U5:34A (snRNA:U5:34A Fly)

Expression

Insect

Promoter

U5:34A

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-cka

Plasmid

#195067

Purpose

Expresses FLAG-tagged Drosophila cka protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Connector of kinase to AP-1 (Cka Fly)

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-wdb

Plasmid

#195069

Purpose

Expresses FLAG-tagged Drosophila wdb protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

widerborst (wdb Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-wrd

Plasmid

#195070

Purpose

Expresses FLAG-tagged Drosophila wrd protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

well-rounded (wrd Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K62D/K64D

Plasmid

#202786

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K62D, K64D

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ACIN1_sgRNA1

Plasmid

#201588

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ACIN1 (ACIN1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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