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We narrowed to 19,275 results for: Tec;

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Showing: 11611 - 11640 of 19275 results

  1. pTRE3G-chimpanzee cGASdeltaN-HA

    Plasmid
    #186874
    Purpose
    Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Chimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal HA tag (CGAS Pan troglodytes, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186874-plasmid-map-sequence-id-366080

    Enlarge

  2. pTRE3G-rhesus macaque cGASdeltaN-HA

    Plasmid
    #186866
    Purpose
    Doxycycline-dependent expression of rhesus macaque cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Rhesus macaque cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (CGAS Macaca mulatta, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186866-plasmid-map-sequence-id-366074

    Enlarge

  4. pTRE3G-orangutan cGASdeltaN-HA

    Plasmid
    #186872
    Purpose
    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186872-plasmid-map-sequence-id-366079

    Enlarge

  5. pTRE3G-human cGASdeltaN-HA

    Plasmid
    #186843
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186843-plasmid-map-sequence-id-366029

    Enlarge

  6. pTRE3G-orangutan cGASdeltaN-GFP

    Plasmid
    #186846
    Purpose
    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186846-plasmid-map-sequence-id-366035

    Enlarge

  7. pTRE3G-chimpanzee cGASdeltaN-GFP

    Plasmid
    #186847
    Purpose
    Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Chimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal GFP tag (CGAS Pan troglodytes, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186847-plasmid-map-sequence-id-366036

    Enlarge

  8. pTRE3G-HA-human cGASdeltaN

    Plasmid
    #186852
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186852-plasmid-map-sequence-id-366437

    Enlarge

  10. pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)

    Plasmid
    #225440
    Purpose
    Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.
    Depositor
    Diego Orzaez
    Article
    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1
    Insert
    nptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225440-plasmid-map-sequence-id-447732

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  11. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

    Plasmid
    #217802
    Purpose
    Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217802-plasmid-map-sequence-id-432882

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  12. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

    Plasmid
    #217806
    Purpose
    Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217806-plasmid-map-sequence-id-432904

    Enlarge

  13. HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

    Plasmid
    #217790
    Purpose
    Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217790-plasmid-map-sequence-id-432853

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  14. HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)

    Plasmid
    #217791
    Purpose
    Variant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    CaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217791-plasmid-map-sequence-id-432854

    Enlarge

  15. HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

    Plasmid
    #217792
    Purpose
    Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217792-plasmid-map-sequence-id-432856

    Enlarge

  16. HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

    Plasmid
    #217793
    Purpose
    Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217793-plasmid-map-sequence-id-432858

    Enlarge

  17. HUHe variant click editor (CE1) - pCMV-T7-SwCV-nCas9-EcKlenow (JO1270)

    Plasmid
    #217794
    Purpose
    Variant CE1 construct with SwCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    SwCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217794-plasmid-map-sequence-id-432859

    Enlarge

  18. HUHe variant click editor (CE1) - pCMV-T7-RaCV-nCas9-EcKlenow (JO1268)

    Plasmid
    #217795
    Purpose
    Variant CE1 construct with RaCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    RaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217795-plasmid-map-sequence-id-432862

    Enlarge

  19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

    Plasmid
    #217799
    Purpose
    Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217799-plasmid-map-sequence-id-432866

    Enlarge

  20. HUHe variant click editor (CE1) - pCMV-T7-BDFV-nCas9-EcKlenow (JO1271)

    Plasmid
    #217785
    Purpose
    Variant CE1 construct with BDFV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    BDFV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217785-plasmid-map-sequence-id-432848

    Enlarge

  21. HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

    Plasmid
    #217786
    Purpose
    Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217786-plasmid-map-sequence-id-432849

    Enlarge

  22. HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

    Plasmid
    #217787
    Purpose
    Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217787-plasmid-map-sequence-id-432850

    Enlarge

  23. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

    Plasmid
    #217788
    Purpose
    Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217788-plasmid-map-sequence-id-432851

    Enlarge

  24. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

    Plasmid
    #217789
    Purpose
    Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217789-plasmid-map-sequence-id-432852

    Enlarge

  26. Quartet-SpyTag

    Plasmid
    #214726
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    Quartet-SpyTag (S Synthetic)
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214726-plasmid-map-sequence-id-435531

    Enlarge

  27. SpyTag-Quartet

    Plasmid
    #214727
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet (S Synthetic)
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214727-plasmid-map-sequence-id-435532

    Enlarge

  28. SpyTag-Alternate Quartet

    Plasmid
    #214728
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Alternate Quartet
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214728-plasmid-map-sequence-id-435533

    Enlarge

  29. SpyTag-Quartet [SARS1]

    Plasmid
    #214729
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet [SARS1] (S Synthetic)
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214729-plasmid-map-sequence-id-435534

    Enlarge

  30. SpyTag-Kraken Quartet

    Plasmid
    #214730
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTag
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Kraken Quartet (S Synthetic)
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214730-plasmid-map-sequence-id-435535

    Enlarge

Showing: 11611 - 11640 of 19275 results