We narrowed to 59,215 results for: {{{s}}}

1. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

   Plasmid

   #172618

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr283Ala

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-YAP-mEGFP

   Plasmid

   #166472

   Purpose

   HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-FLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172619

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FUW-Flag-Mbd3 delta1-70

   Plasmid

   #52372

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-70

   Promoter

   Ubiquitin

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LV-AR-R599A-N600A

   Plasmid

   #171221

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-R599A-N600A (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-R599A-N600A

   Promoter

   EF-1a

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYeDP60-hATP13A2-E343A

   Plasmid

   #171378

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Use

   Tags

   BAD tag

   Expression

   Yeast

   Mutation

   E343A

   Promoter

   URA3

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

   Plasmid

   #170197

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

   Promoter

   CMV51

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FUW-Flag-Mbd3 delta49-63

   Plasmid

   #52374

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 49-63

   Promoter

   Ubiquitin

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCG_SARS-CoV2 NSP15 C-V5-tag

   Plasmid

   #179975

   Purpose

   Mammalian expression vector for SARS-CoV-2 Nsp15, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-2 Nsp15 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDF1-WT_RASA2-EF1-Puro

    Plasmid

    #114096

    Purpose

    Express RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LV-AR-AAFF

    Plasmid

    #171218

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-AAFF (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-K580A-V581A

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mEGFP-N1-MST2

    Plasmid

    #166475

    Purpose

    Expresses fusion of mEGFP and MST2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, MST2 (STK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-PGK-RAP1A-E63-puro

    Plasmid

    #156167

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. A*03:02:01

    Plasmid

    #203294

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*03:02:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQCXIZ GFP PODXL 4K>R

    Plasmid

    #202420

    Purpose

    Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    K431/525/526/547R

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

    Plasmid

    #172647

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-PGK-RAP1A-WT-neo

    Plasmid

    #156173

    Purpose

    Lentiviral vector for expression of RAP1A-WT, with neomycin section

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-FLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172626

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B*58:02:01

    Plasmid

    #203262

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:02:01 (HLA-B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

    Plasmid

    #170198

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge