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OPEN-HLA-B*07:02-BirA

Plasmid

#239752

Purpose

E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-B*07:02 (HLA-B Human)

Tags

BirA substrate peptide

Expression

Bacterial

Mutation

G120C

Promoter

T7

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-S170D

Plasmid

#213592

Purpose

Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

S170D

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/D338N

Plasmid

#213594

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, D338N

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pInducer-myc-ERK1-R84S/S170D/D338N

Plasmid

#213595

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, D338N

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D/Y280A

Plasmid

#213596

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, Y280A

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/Y263A

Plasmid

#213599

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, Y263A

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB398_ZFcharm_Prnp_SMM

Plasmid

#220845

Purpose

AAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding site

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB303_ZFcharm_Kv1_Prnp_SMM

Plasmid

#220841

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single mismatch self-silencing binding site

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah

Plasmid

#199264

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

Depositor

Erik Sontheimer

Publication

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5-toku (1-174)

Plasmid

#134261

Purpose

Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-174

Promoter

CMV

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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SLF063: Lenti-fCAG-jRGECO1a

Plasmid

#124775

Purpose

For general lentiviral or direct expression of cytosolic jRGECO1a

Depositor

Adam Cohen

Publication

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

jRGECO1a

Use

Lentiviral

Tags

NES-6xHIS

Expression

Mammalian

Promoter

CAG

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCMV4a-SIRT6-FLAG

Plasmid

#102322

Purpose

Human SIRT6 with FLAG tag for mammalian expression

Depositor

Hening Lin

Publication

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Rap2b

Plasmid

#118321

Purpose

Expression of human Rap2b

Depositor

Philip Stork

Publication

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap2b (RAP2B Human)

Tags

GFP

Expression

Mammalian

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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WT C-His Ana GvpC in pET28a

Plasmid

#85732

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Publication

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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Lentiviral CaMKKbeta

Plasmid

#33322

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Promoter

human PGK

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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sh-RNA hULK2

Plasmid

#31970

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pEXSISERS_FLuc_loxP-PuroR

Plasmid

#177866

Purpose

Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Publication

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

FLuc-based EXSISERS

Use

CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

Tags

FLAG

Expression

Mammalian

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pUSE-RapR-Src(KD)-myc

Plasmid

#25934

Depositor

Klaus Hahn

Publication

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-Src (Fkbp1a Mouse)

Expression

Mammalian

Mutation

This pUSE-RapR-Src(KD)-myc plasmid contains the k…

Available since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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