Addgene

We narrowed to 6,474 results for: sas

Showing: 761 - 780 of 6474 results

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pSMP-SMYD2_2

Plasmid

#36350

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
p068 pCS mTRPS1 (M)

Plasmid

#11040

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Two Cys residues at positions 917 and 920 in the …

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

Plasmid

#126026

Purpose

express RFP-tagged LARIAT variant for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHRolig-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ratFDH

Plasmid

#135813

Purpose

Expresses rat FDH (also referred to as AldH1L1) in mammalian cells.

Depositor

Sergey Krupenko

Article

Krupenko et al Cell Growth Differ. 2002 May;13(5):227-3

Insert

Aldh1l1 (Aldh1l1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCaSpeR3/ncd-gfp #10

Plasmid

#66082

Purpose

stable germline transformation of Drosophila to produce Ncd-GFP expression

Depositor

Sharyn Endow

Article

Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

Insert

Ncd kinesin-14 motor protein (ncd Fly)

Use

Tags

GFP

Expression

Insect

Mutation

D699L

Promoter

ncd promoter (3.1kb upstream region of ncd start …

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

Plasmid

#69223

Purpose

Expresses R1335K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126025

Purpose

express RFP- and mCer-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-SMYD2_3

Plasmid

#36351

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
mEos2-CENPB-N-22

Plasmid

#57360

Purpose

Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPB (CENPB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-EB1-C-18

Plasmid

#57371

Purpose

Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

EB1 (MAPRE1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Dectin1A-C-10

Plasmid

#57367

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Dectin1A (CLEC7A Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Dectin1A-N-10

Plasmid

#57368

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Dectin1A (CLEC7A Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

Plasmid

#233067

Purpose

Piggybac CRISPRi construct

Depositor

Markus Landthaler

Article

Vučićević et al bioRxiv 2024.09.12.612198

Insert

KRAB-dCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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BtA1N

Plasmid

#238979

Purpose

In vitro transcription of bovine arrestin-1

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-1 (SAG Bovine)

Use

In vitro transcription

Tags

Expression

Mutation

silent

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2N

Plasmid

#238980

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Tags

Expression

Mutation

silent

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 (1-378)

Plasmid

#236264

Purpose

Bacterial expression of bovine arrestin-1 (1-378) E379Stop

Depositor

Vsevolod Gurevich

Insert

arrestin-1 (SAG Bovine)

Use

Tags

Expression

Bacterial

Mutation

(1-378) E379Stop, inserted GGT codon after starti…

Promoter

lac

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 R175E

Plasmid

#236263

Purpose

Bacterial expression of bovine arrestin-1 R175E with inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Insert

arrestin-1 (SAG Bovine)

Use

Tags

Expression

Bacterial

Mutation

R175E, inserted GGT codon after starting ATG

Promoter

lac

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1a-FLAG

Plasmid

#232143

Purpose

Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

Zebrafish expression

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-dgat1b in situ probe

Plasmid

#232138

Purpose

zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

Plasmid

#193050

Purpose

Encodes guide RNA expression targeting PX740 landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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