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Showing: 761 - 780 of 1427 results

  1. pV1081

    Plasmid
    #111425
    Purpose
    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgADE-NT2
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111425-plasmid-map-sequence-id-212268

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  2. pDual_dsCas9_Venus_hs_NRF2

    Plasmid
    #214692
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human NRF2
    Depositor
    Kevin Janes
    Insert
    dgRNA_NRF2 (NFE2L2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214692-plasmid-map-sequence-id-428724

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  3. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid
    #104438
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104438-plasmid-map-sequence-id-201144

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  7. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164265-plasmid-map-sequence-id-330127

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  8. pXPR_071

    Plasmid
    #164558
    Purpose
    Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164558-plasmid-map-sequence-id-330194

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  10. pHR-UCOE-SFFV-dCas9-P2A-EGFP

    Plasmid
    #188898
    Purpose
    Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS and P2A-GFP
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188898-plasmid-map-sequence-id-374870

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  14. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid
    #64219
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP-P2A-E1B
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64219-plasmid-map-sequence-id-107960

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  15. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, H1, and U6
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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  16. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  17. LentiRCas9-Lambda2

    Plasmid
    #104184
    Purpose
    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-sgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104184-plasmid-map-sequence-id-201745

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  18. pXPR_068

    Plasmid
    #164555
    Purpose
    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164555-plasmid-map-sequence-id-330129

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  19. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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Showing: 761 - 780 of 1427 results