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pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107322

Purpose

Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to SaCas9

Promoter

CMV IE93

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107325

Purpose

Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to NmCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107326

Purpose

Expresses SpCas9 (R1333S) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to NmCas9

Promoter

CMV IE93

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_nucl

Plasmid

#113928

Purpose

Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_nucl

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGex4t2 with full-length SUUR

Plasmid

#112990

Purpose

Expresses fusion of GST and full-length Drosophila SUUR

Depositor

Terry Orr-Weaver

Article

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse aB crystallin 800bp promoter/AcGFP

Plasmid

#98103

Purpose

Mouse aB crystallin 800bp promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Promoter

Alpha B-crystallin promoter 800 bp fragment (-833…

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Danio alpha Ba-crystallin 3kb promoter/AcGFP

Plasmid

#98097

Purpose

aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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hFlt1 Y1333F

Plasmid

#86037

Purpose

human VEGFR1 with Y to F mutation at position 1333

Depositor

Christie Thomas

Article

Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

Insert

human Flt1 Y1333F (FLT1 Human)

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

Y1333F; HA tag is inserted downstream of signal …

Promoter

CMV

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1604

Plasmid

#78194

Purpose

Ce cha-1 expression in motor neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

unc-3

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
AEB-Theo27 pUC19

Plasmid

#71419

Purpose

This pUC19 vector contains a theophylline binding riboswitch, controlling the translation initiation rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

Theo27 theophylline riboswitch

Use

Synthetic Biology

Promoter

ptac

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pFPL-Yg

Plasmid

#61655

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 Mor2 shRNA

Plasmid

#59298

Purpose

3rd generation lentiviral vector expressing shRNA to mouse Opmr1

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Mor2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D RNAiR

Plasmid

#51600

Purpose

expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations between amino acids (approx 275-…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ

Plasmid

#27424

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pBABEhygro-Flag-ZO-2-delta-N

Plasmid

#27476

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pET-21a(+)-T-protein

Plasmid

#12652

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2004 Nov 26. 279(48):50514-

Insert

T-protein

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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