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pMaCTag-Z24

Plasmid

#120067

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z10

Plasmid

#120053

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z01

Plasmid

#120044

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P16

Plasmid

#120027

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mRuby-N

Plasmid

#113752

Purpose

Gateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmEGFP-N

Plasmid

#113747

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmEGFP-N

Plasmid

#113748

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

3xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmEGFP-C

Plasmid

#113750

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmRuby-N

Plasmid

#113753

Purpose

Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

2xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmRuby-C

Plasmid

#113756

Purpose

Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

2xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

Plasmid

#87572

Purpose

PV4 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

Plasmid

#89056

Purpose

PV3 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch5_155183064-gRNA-rev

Plasmid

#81215

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-for2

Plasmid

#81209

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pUNC2001

Plasmid

#67280

Purpose

Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

Depositor

Aziz Sancar

Article

Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

Insert

Vibrio cholera photolyase

Tags

maltose binding protein

Expression

Bacterial

Mutation

none known

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 R4-R3

Plasmid

#62105

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_PTS2

Plasmid

#65675

Purpose

Expresses PTS2-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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ROZA YF

Plasmid

#64197

Purpose

Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA YF

Expression

Mammalian

Mutation

tyrosine phosphorylatin site mutated to phenylala…

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 L1-L4

Plasmid

#62104

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L1-R5

Plasmid

#62101

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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