We narrowed to 19,051 results for: Tec

1. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

   Plasmid

   #200904

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB_NE1m

   Plasmid

   #123308

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1m
   

   Use

   AAV

   Mutation

   contains a glycine-to-threonine mutation at posit…

   Promoter

   hSyn

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPIH6

   Plasmid

   #190676

   Purpose

   E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

   Depositor

   John Vederas

   Article

   Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

   Inserts

   Small ubiquitin-like modifier
   Mxe GyrA intein with C-terminal chitin binding domain and His tag
   

   Tags

   Chitin binding domain, His tag, and N-terminal Hi…

   Expression

   Bacterial

   Mutation

   Added C-terminal his tag and None

   Promoter

   T7

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

   Plasmid

   #83919

   Purpose

   Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   DsRed-Express2
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Promoter

   hUbC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSK021 CMV-TO-p53R248Q (FLP-IN)

   Plasmid

   #191167

   Purpose

   Expression of human p53 mutant R248Q in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248Q) (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R248Q

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK322 (TOP2A-mAC (Hygro))

   Plasmid

   #140653

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGLO-GFP-1UAG

   Plasmid

   #82500

   Purpose

   Expresses GFP with 1 UAG codon at the amino acid position 3

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescent Protein with 1 UAG codon
   

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3

   Promoter

   AraBAD

   Available Since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINTO-NFH::hSNRNP70

   Plasmid

   #65926

   Purpose

   Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Two silent mutations

   Promoter

   CMV/TO and CMV/TetO2 (T-REx)

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKb-TnpB2

    Plasmid

    #215334

    Purpose

    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

    Depositor

    Kutubuddin Molla

    Article

    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

    Insert

    Rice codon optimized ISDra2 TnpB
    

    Tags

    Not applicable

    Expression

    Plant

    Promoter

    Rice ubiquitin promoter for TnpB and maize ubiqui…

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30a-His6-LOXCAT

    Plasmid

    #140084

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. A*68:01:01

    Plasmid

    #203292

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*68:01:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpRY-ABE8e(V106W)

    Plasmid

    #198553

    Purpose

    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-ms-Klf2

    Plasmid

    #50786

    Purpose

    retroviral expression of mouse Klf2

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Klf2 (Klf2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti NL

    Plasmid

    #113450

    Purpose

    Lentiviral NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. A*02:11:01

    Plasmid

    #203280

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*02:11:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid

    #181743

    Purpose

    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA183

    Plasmid

    #216025

    Purpose

    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9 (H840A)_v2.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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