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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-3HA-SREBP1-C-Flag

Plasmid

#134286

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP1 (Srebf1 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP1 isoform a precursor

Promoter

EF1a

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v1

Plasmid

#134275

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

524 amino acids, isoform 1

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v2

Plasmid

#134277

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

553 amino acids, isoform 2

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DM1-3_H0-ABD+

Plasmid

#234554

Purpose

CTNNA1 M-domain deletion mutant with enhanced actin-binding domain mutations

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DM-domain-H0-ABD+ (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v1

Plasmid

#134270

Purpose

Lentivector encoding Flag-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 1; 524 amino acids

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v2

Plasmid

#134271

Purpose

Lentivector encoding Flag-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 2; 553 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v2

Plasmid

#134269

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v1

Plasmid

#134268

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v1

Plasmid

#134257

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

Plasmid

#134252

Purpose

Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

deleted amino acids 179-219, and mutated coding s…

Promoter

EF1a

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-EGFP-PARP1

Plasmid

#176146

Purpose

EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

Plasmid

#176150

Purpose

N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

Mutation in the PAM site used by POLB gRNA1

Promoter

EF1A

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mNeurog2

Plasmid

#198754

Purpose

Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Neurogenin-2 (Neurog2 Mouse)

Use

Lentiviral

Promoter

TRE3G

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mAscl1

Plasmid

#198755

Purpose

Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Promoter

TRE3G

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK (281-473)

Plasmid

#134273

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 281-473

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK (12-272)

Plasmid

#134272

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 12-272

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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