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pET22b-ecDHFR-D27S/Y100F

Plasmid

#110827

Purpose

Bacterial expression of E.coli DHFR D27S/Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Expression

Bacterial

Mutation

D27 mutated to S27, Y100 mutated to F100

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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AY23_pgRNA.R5.2

Plasmid

#100295

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6 Polymerase III snRNA

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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GST-RP2-C86Y,P95L

Plasmid

#86074

Purpose

A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed cysteine-86 and proline-95 to tyrosine an…

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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GST-cystin-CTS

Plasmid

#86066

Purpose

A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Cystin (Cys1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 28-35

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

Plasmid

#61796

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

Plasmid

#61793

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

Plasmid

#61791

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

Plasmid

#61790

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

Plasmid

#61786

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLexA::SRDX (GB0732)

Plasmid

#68184

Purpose

Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LexA Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H09

Plasmid

#71003

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

SNF4Agamma (SNF4Agamma Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C07

Plasmid

#70985

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

14-3-3zeta (14-3-3zeta Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2B09

Plasmid

#70918

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

gro (gro Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C03

Plasmid

#70959

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf4 (Taf4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pGal4BD::BDR (GB0734)

Plasmid

#68185

Purpose

Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Gal4 Binding Domain fused to BDR Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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CC1

Plasmid

#63713

Purpose

Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

CC1

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR SFFV tdTomato L5-L2

Plasmid

#62155

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SFFV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV iRFP L3-L2

Plasmid

#62159

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 PuroR L5-L2

Plasmid

#62165

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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