We narrowed to 18,501 results for: MUT

1. pDONR223_ZNF597_WT

   Plasmid

   #82894

   Purpose

   Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   ZNF597 (ZNF597 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG CMV14 / BRAF-G596C

   Plasmid

   #131712

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G596C

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xFLAG CMV14 / BRAF-G469S

   Plasmid

   #131716

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G469S

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG CMV14 / BRAF-V600R

   Plasmid

   #131721

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600R

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT22Ubm5En2SRE4PCh(#302)

   Plasmid

   #184089

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-chkEn2(SR)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDNA2.0 6H-TEV-KRAS

   Plasmid

   #111849

   Purpose

   Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

   Depositor

   Kenneth Westover

   Article

   Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

   Insert

   KRAS (KRAS Human)
   

   Tags

   6xHis-tag and TEV protease cleavage sequence

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-ccdB-boxB-tBE-V5-mA3

   Plasmid

   #171693

   Purpose

   Expresses tBE-V5-mA3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 and EF1a

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

   Plasmid

   #173029

   Purpose

   For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   pspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, BFP, HIV NES, and T2A

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-puro /BRAF-V600E

    Plasmid

    #131724

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    CMV

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-PDGFRB

    Plasmid

    #116770

    Purpose

    Lentiviral expression of PDGFRB

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid

    #226105

    Purpose

    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    atf4 (ATF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains uORF1-2 of ATF4, whose stability can ser…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-RET

    Plasmid

    #116787

    Purpose

    Lentiviral expression of RET

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RET (RET Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-B2M

    Plasmid

    #116715

    Purpose

    Lentiviral expression of B2M

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    B2M (B2M Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHEE401E

    Plasmid

    #71287

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-PIK3CA

    Plasmid

    #116771

    Purpose

    Lentiviral expression of PIK3CA

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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