We narrowed to 18,615 results for: yeast expression

1. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

   Plasmid

   #207020

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   I375A

   Promoter

   pTEF1

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

   Plasmid

   #207019

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A

   Promoter

   pTEF1

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ClhN-P1K-3VSV-Atg8-MET15

   Plasmid

   #207055

   Purpose

   Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xVSV

   Expression

   Yeast

   Promoter

   pATG8

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. P1K-3HA-Atg8∆GR-MET15

   Plasmid

   #207033

   Purpose

   Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆GR

   Promoter

   pATG8

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBT9-γ1 ear

   Plasmid

   #199177

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   AP-1 γ1 ear domain (Ap1g1 Mouse)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAD424-Rabex-5

   Plasmid

   #197043

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   Rabex-5 (RABGEF1 Bovine)
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT175_GalL_A3A(Y130F)Δ186-BE3

   Plasmid

   #145124

   Purpose

   Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)Δ186-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(Y130F; 1-186AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Tags

    9xMyc

    Expression

    Yeast

    Promoter

    pADH1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT957

    Plasmid

    #228293

    Purpose

    Constitutive mutant PhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    phlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    E41G

    Promoter

    ScPGK1 promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT1035

    Plasmid

    #228300

    Purpose

    Constitutive expression of LuxTA mutant with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    luxTA mutant
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal-Three copies of VP16 …

    Expression

    Yeast

    Mutation

    S116Y, W201R

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDC175

    Plasmid

    #103019

    Purpose

    S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJZC638

    Plasmid

    #62283

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been deactivated by mutatio…

    Promoter

    pAdh and pGal10

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p415TEF cyto roGFP(iL)-Grx1(human)

    Plasmid

    #65002

    Purpose

    Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    roGFP(iL)

    Expression

    Yeast

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Expression

    Bacterial and Yeast

    Promoter

    TDH3

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUDR211

    Plasmid

    #113870

    Purpose

    expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA3-HXT8 / sgRNA4-HXT14
    

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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