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pLVX-Flag-Polß(TM-KTM)-IRES-neo

Plasmid

#128675

Purpose

mammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R-K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM/K206A/K244A)-IRES-puro

Plasmid

#128684

Purpose

mammalian expression of Flag-Polß(TM/K206A/K244A: L301R/V303R/V306R/K206A/K244A)) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag tag

Expression

Mammalian

Mutation

L301R/V303R/V303R/K206A/K244A

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAav-TP53-PQS1-3xFLAG

Plasmid

#84884

Purpose

rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

TP53 genomic region HR1 (TP53 Human)
TP53 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no and no

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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RF21

Bacterial Strain

#102803

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF12

Bacterial Strain

#62077

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML2

Bacterial Strain

#61906

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML6

Bacterial Strain

#61908

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE, avtA genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pHLsec-FAP

Plasmid

#239817

Purpose

Expresses human fibroblast activation protein alpha (FAP) in mammalian cells

Depositor

Ingrid De Meester

Article

Verhulst et al Eur J Med Chem. 2026 Feb 15;304:118494.

Insert

Fibroblast activation protein alpha (FAP Human)

Tags

8xHIS tag and TwinStrep tag

Expression

Mammalian

Mutation

No mutations

Promoter

Chicken beta-actin

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-SEP-GluA1

Plasmid

#227235

Purpose

Large cargo capacity AAV that expresses SEP tagged AMPA receptor subunit GluA1 in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

SEP-GluA1 (Gria1 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10

Plasmid

#180348

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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