We narrowed to 43,429 results for: Ina

1. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

   Plasmid

   #214265

   Purpose

   Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

   Depositor

   D. James Surmeier

   Insert

   Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Synapsin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-PKI nls

   Plasmid

   #118301

   Purpose

   Expression of PKIa

   Depositor

   Philip Stork

   Article

   Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

   Insert

   PKI nls (aa 2-36)
   

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   inserting 2xnls

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV3Tag8li-hDDX41-HA

   Plasmid

   #165469

   Purpose

   Human DDX41 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   DEAD-box helicase 41 (DDX41 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM512

   Plasmid

   #140192

   Purpose

   For curing of Orbit-integrated plasmids and associated drug marker

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   zeocin resistance gene
   Bxb1gp47 (directionality factor)
   

   Expression

   Bacterial

   Mutation

   Cloned in gp47 directinality factor in place of R…

   Promoter

   EM7 and Ptet

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
   

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

   Plasmid

   #123362

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Firefly luciferase
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

   Plasmid

   #26966

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   ER export at 3' end of gene. Trafficking sig…

   Promoter

   EF1alpha

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV500 A-CREB

   Plasmid

   #33371

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   CREB (Creb1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available Since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-opto-hCaspase-1

   Plasmid

   #208790

   Purpose

   Inducibly expresses human Caspase1, fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Caspase1 (CASP1 Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZW6 (CK2alpha)

    Plasmid

    #27086

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM-AAV-mSncg-0.27kb-EGFP

    Plasmid

    #153185

    Purpose

    Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-POMP

    Plasmid

    #86765

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human POMP (POMP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKrox24(2xD-E)DsRed

    Plasmid

    #200111

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-opto-hCaspase-4 dCARD

    Plasmid

    #208785

    Purpose

    Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry Caspase4 dCARD (CASP4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ExRai-AktAR2

    Plasmid

    #184047

    Purpose

    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    ExRai-AktAR2
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-Padron2

    Plasmid

    #172355

    Purpose

    Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

    Depositor

    Stefan Jakobs

    Article

    Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

    Insert

    Padron2
    

    Tags

    6xHisTag (N terminal on backbone)

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-GfaABC1D-ERCreER-4x6T

    Plasmid

    #196411

    Purpose

    Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    ERT2-Cre-ERT2
    

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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