We narrowed to 19,051 results for: Tec

1. pCEP4-HA-Flag-natT1R2 FS1

   Plasmid

   #113955

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, one base pair dele…

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. eTd-CBEm

   Plasmid

   #196602

   Purpose

   Mammalian expression of eTd-CBEm

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_Anc689-NG-nCas9

   Plasmid

   #163527

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP2133

   Plasmid

   #72249

   Purpose

   Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y450A, N497A, R661A, Q695A, and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SFFV-HALO-NTERM-GFP

   Plasmid

   #185762

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF-MjCouRS

   Plasmid

   #229066

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   Methanococcus jannaschii tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

   Plasmid

   #69625

   Purpose

   A FRET sensor for Galphai3 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Inserts

   Gbeta-2A-cpV-Ggamma2
   Galphai3-mTurquoise2
   

   Tags

   ciruclar permutated Venus (cp173V) and mTurquoise…

   Expression

   Mammalian

   Promoter

   CMV and CMV (IRES)

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGK-AID-NTERM-GFP

   Plasmid

   #185773

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, AID

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK4-3XFlag-pBabe

   Plasmid

   #73558

   Purpose

   vector encoding a fusion protein between CDK4 and 3 Flag tags

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Retroviral

   Tags

   3X-FLAG

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mKate2-P2A-APEX2-2xFYVE_hrs

    Plasmid

    #67663

    Purpose

    Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-2xFYVE
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_Xph20-GCaMP7f

    Plasmid

    #135539

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    GCaMP7f

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. eTd-CBEa

    Plasmid

    #196603

    Purpose

    Mammalian expression of eTd-CBEa

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Tags

    6*His

    Expression

    Mammalian

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SFFV-HALO-CTERM-GFP

    Plasmid

    #185767

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-GCaMP6s

    Plasmid

    #183809

    Purpose

    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene TetO promoter-DIO/flex-GCaMP6
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAPM-miR30-DDX58-ts3

    Plasmid

    #174243

    Purpose

    DDX58 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    DDX58 shRNA (RIGI Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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