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Plasmid#192159PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
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WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204798PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204799PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204797PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-itis Bacterial Base Editor Activity
Plasmid Kit#1000000219PurposeThis collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.DepositorAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDB_345
Plasmid#231113PurposeABE activity-based selection cloning vectorDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDB_347
Plasmid#231114PurposeCBE activity-based selection cloning vectorDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA_867
Plasmid#231109PurposeA>G base editorDepositorInsertABE8e-nCas9-SpG-HA
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA566
Plasmid#231115PurposeFragmid fragment: (guide cassette) ABE activity-based selection cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA568
Plasmid#231117PurposeFragmid fragment: (guide cassette) CBE activity-based selection cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_TUB4S351F_1397NC
Plasmid#193382PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtTUB4.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
AYBEv0.1
Plasmid#193966PurposeA wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(wt)
UseTagsExpressionMammalianMutationPromoterhU6, EF1a, CBhAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDB_092
Plasmid#231110PurposeC>T base editorDepositorInsertTadA-CDd-nCas9-SpG-UGI
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDB_270
Plasmid#231111PurposeC>T base editorDepositorInsertTadA-CDd-nCas9-SpG-UGI-HA
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA516
Plasmid#231118PurposeFragmid fragment: (2A Selection) CBE activity-based selection intronDepositorInsertPuroN'-intron-PuroC'
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA498
Plasmid#231119PurposeFragmid fragment: (guide cassette) ABE activity-based selection CD274 positive controlDepositorInsertsgCD274 + ABE splice-targeting sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA505
Plasmid#231120PurposeFragmid fragment: (guide cassette) CBE activity-based selection CD274 positive controlDepositorInsertsgCD274 + CBE splice-targeting sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA509
Plasmid#231116PurposeFragmid fragment: (2A Selection) ABE activity-based selection intronDepositorInsertPuroN'-intron-PuroC'
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
nTALE+CD_for TUB_8N_1397NC
Plasmid#193383PurposenTALECD (1397NC) expression vectorDepositorInsertFor multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_CYO1 exon1_1397NC
Plasmid#193376PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only