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pAAV-synapsin-HaloCaMP1b-EGFP (AAV1)

Viral Prep

#138328-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-synapsin-HaloCaMP1b-EGFP (#138328). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1b-EGFP plasmid DNA. Synapsin-driven HaloCaMP1b, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Promoter

Syn

Tags

EGFP

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-synapsin-HaloCaMP1a-EGFP (AAV9)

Viral Prep

#138327-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-synapsin-HaloCaMP1a-EGFP (#138327). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1a-EGFP plasmid DNA. Synapsin-driven HaloCaMP1a, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Promoter

Syn

Tags

EGFP

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-synapsin-HaloCaMP1b-EGFP (AAV9)

Viral Prep

#138328-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-synapsin-HaloCaMP1b-EGFP (#138328). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1b-EGFP plasmid DNA. Synapsin-driven HaloCaMP1b, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Promoter

Syn

Tags

EGFP

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Expression

Mutation

Promoter

SFFV

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

Plasmid

#169338

Purpose

Expression of the LT-Fucci(CA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(CA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET12a-HAL

Plasmid

#47097

Depositor

Marina Ramirez-Alvarado

Insert

HAL

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

Plasmid

#169337

Purpose

Expression of the LT-Fucci(SCA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SCA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-ER-3

Plasmid

#186960

Purpose

ER lumen localizaing HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CALR (CALR Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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DCTN1_Halo_C_allele

Plasmid

#178156

Purpose

Donor vector for endogenous tagging of human DCTN1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (DCTN1 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_Halo_GluA2

Plasmid

#170454

Purpose

Expresses halo tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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OTUD4_Halo_C_allele

Plasmid

#178164

Purpose

Donor vector for endogenous tagging of human OTUD4 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (OTUD4 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Huaiying Zhang, Michael Lampson

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_HaloK73T_cyto

Plasmid

#183687

Purpose

Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

Cyto Halo(K73T)

Use

Tags

Expression

Mammalian

Mutation

K73T in Halotag

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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