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Plasmid#51272PurposeComponent of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)DepositorInsertNLS-HA-Dre
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Co-InCreN
Plasmid#51267PurposeThe N-terminal component of the Co-InCre system, expresses the N-Cre-N-gp41-1 fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreCDepositorInsertCo-InCreN
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Co-InCreC
Plasmid#51268PurposeThe C-terminal component of the Co-InCre system, expresses the C-gp41-1-C-Cre fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreNDepositorInsertCo-InCreC
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-NLS-HA-Dre-P2A-BFP
Plasmid#51277PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoterDepositorInsertNLS-HA-Dre-P2A-BFP
UseTagsExpressionMutationPromoterhGFAPAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-attBr-ZsGreen-attP
Plasmid#51265PurposeBxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientationDepositorInsertattBr-ZsGreen-attP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EYFP,attP}
Plasmid#86860PurposeExpresses EYFP in eyes and contains an attP landing site for site-specific attB integrationDepositorInsertattP
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
p{CFD4-EYFP-3xP3::DsRed}
Plasmid#86863PurposeExpresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-attBr-Cre-rc-attP
Plasmid#51264PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientationDepositorInsertattBr-Cre-rc-attP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p{CFD4-3xP3::DsRed}
Plasmid#86864PurposeExpresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSAR-Cre-F2A-I-SceI
Plasmid#62302PurposeAnopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed markerDepositorInsertCre recombinase, I-SceI nuclease
UseTagsExpressionInsectMutationPromotervasaAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::DsRed, Pactin::Ptrsps}
Plasmid#86862PurposeExpresses DsRed in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
UseTagsExpressionInsectMutationS129APromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4-vas2-integrase-R3
Plasmid#62299PurposephageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoterDepositorInsertphageC31 integrase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-vas2-Cre-L4
Plasmid#62301PurposeCre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoterDepositorInsertCre recombinase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
CC1215
Bacterial Strain#79062PurposeBL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.DepositorBacterial ResistanceKanamycinAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
p1242 3x-KB-L
Plasmid#26699DepositorInsert3x MHC I KB element - luciferase (NFKB1 Human, Photinus pyralis)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-p105
Plasmid#23288DepositorInsertp105 (NFKB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only