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Showing: 61 - 80 of 13344 results

  1. Double UP mNeon to mScarlet Rac1-V12

    Plasmid
    #125136
    Purpose
    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).
    Depositor
    Erik Dent
    Article
    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d
    Insert
    mNeon/mScarletP2A Rac1-V12
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed Not1 site in mScarlet. 12th AA in Rac1 ch…
    Promoter
    CAG
    Available since
    May 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    125136-plasmid-map-sequence-id-244760

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  2. Double UP mNeon to mScarlet Rac1-N17

    Plasmid
    #125137
    Purpose
    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).
    Depositor
    Erik Dent
    Article
    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d
    Insert
    mNeon/mScarletP2A Rac1-N17
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed Not1 site in mScarlet. 17th AA in Rac1 ch…
    Promoter
    CAG
    Available since
    May 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    125137-plasmid-map-sequence-id-244754

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  3. pMflT-o2

    Plasmid
    #101310
    Purpose
    Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101310-plasmid-map-sequence-id-198875

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  4. pMflT-o1

    Plasmid
    #101309
    Purpose
    Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101309-plasmid-map-sequence-id-198143

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  5. pMflET-o4

    Plasmid
    #101314
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101314-plasmid-map-sequence-id-198386

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  6. pTE1059

    Plasmid
    #186628
    Purpose
    Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.
    Depositor
    Eriko Takano
    Article
    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10
    Inserts
    scbA
    scbB
    scbC
    Use
    Synthetic Biology
    Tags
    6xHis and 6xHis-thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic promoter B10, Synthetic promoter B19, a…
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    186628-plasmid-map-sequence-id-371218

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  7. pMflT-o4

    Plasmid
    #101312
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101312-plasmid-map-sequence-id-198865

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  8. pMflPT-o4

    Plasmid
    #101315
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101315-plasmid-map-sequence-id-198861

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  9. pMflST-o4

    Plasmid
    #101316
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101316-plasmid-map-sequence-id-199230

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  10. pMflT-o3

    Plasmid
    #101311
    Purpose
    Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101311-plasmid-map-sequence-id-198141

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  11. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Use
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  12. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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  14. pCHAP3_Sap

    Plasmid
    #206849
    Purpose
    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206849-plasmid-map-sequence-id-419973

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  17. pCHAP3_URA

    Plasmid
    #206848
    Purpose
    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206848-plasmid-map-sequence-id-419965

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Showing: 61 - 80 of 13344 results