Addgene

We narrowed to 1,297 results for: sds

Showing: 61 - 80 of 1297 results

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pCYC1m_yeGFP

Plasmid

#64389

Purpose

encodes yeast enhanced GFP under control of minimal pCYC1 promoter

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pCYC1

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pBI-GFP

Plasmid

#16623

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

Plasmid

#81253

Purpose

Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

TetR and LacI

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA

Plasmid

#81254

Purpose

For expressing Dox and IPTG inducible stgRNAs

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

stgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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1x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64390

Purpose

encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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PCLP-2/pCMV (100)

Plasmid

#24421

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pEF6V5:eGFP-CAAX-2A-mCherry

Plasmid

#26901

Depositor

Steven Leach

Article

Provost et al Genesis. 2007 Oct . 45(10):625-9.

Insert

eGFP-CAAX-PTVI-2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16a

Plasmid

#31503

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRPR1(1xTetO)_gRNA_handle_RPR1t

Plasmid

#62966

Purpose

aTc inducible gRNA expression vector

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16df

Plasmid

#31505

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16d/f specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pBM5218

Plasmid

#18796

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Ty5-HIS3AI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
L4440-daf-16abf

Plasmid

#31507

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/b/f common (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pET3_HMV-His6

Plasmid

#164843

Purpose

Expression of FL human metavinculin

Depositor

Tina Izard

Article

Rangarajan et al PLoS One. 2010 May 19;5(5):e10679. doi:

Insert

VCL (VCL Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16b

Plasmid

#31504

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16b specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

Plasmid

#81246

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16af

Plasmid

#31506

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/f common (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
Construct 1 - U6p_20nt1_WT_sgRNA

Plasmid

#81245

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
12x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64393

Purpose

encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

pCYC1

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only