We narrowed to 1,143 results for: fret

1. pRSETA_crE6G2

   Plasmid

   #172929

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6G2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Val317Gly, Met316Gly

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA-FRT-FAK0

   Plasmid

   #45047

   Purpose

   FAK fret sensor without helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-iGECInano

   Plasmid

   #186189

   Purpose

   Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

   Insert

   iGECInano
   

   Expression

   Bacterial

   Promoter

   AraC

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Golgi-DKAR

   Plasmid

   #49790

   Purpose

   FRET-based, Golgi-targeted kinase activity reporter for PKD

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

   Insert

   D kinase activity reporter
   

   Tags

   CFP, YFP, and eNOS(1-33)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ypet-RBD

   Plasmid

   #105291

   Purpose

   To measure Rho activity by binding and FRET analysis

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   RBD (Rtkn Mouse)
   

   Tags

   Ypet

   Expression

   Mammalian

   Mutation

   aa encoding 7-89

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-FAK20

   Plasmid

   #59130

   Purpose

   Cloning vector for FAK fret sensor with 20 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB.DEST-Illusia

   Plasmid

   #215444

   Purpose

   Piggybac expression vector with Illusia FRET reporter for integrin phosphorylation

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   Illusia
   

   Use

   Transposon-based stable expression

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pRSETA_crG18

    Plasmid

    #172930

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorG18
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-AKAR4-Kras

    Plasmid

    #61621

    Purpose

    Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4-Kras
    

    Tags

    C-terminal targeting sequence from Kras, Cerulean…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 hSynapsin1 NAPA-N SV40

    Plasmid

    #92282

    Purpose

    FRET probe; extracellular synapse targeted mCherry

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Promoter

    hSynapsin1

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTet_NADP-Snifit-mito

    Plasmid

    #113919

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Cerulean-Epac2-Venus

    Plasmid

    #138196

    Purpose

    FRET-based biosensor for monitoring Epac2 activation.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Clover-Prx2-mRuby2

    Plasmid

    #117906

    Purpose

    fluorescent reporter of cytosolic hydrogen peroxide

    Depositor

    Hadley Sikes

    Article

    Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

    Insert

    Clover-Prx2-mRuby2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Ras-LOCKR-S

    Plasmid

    #216496

    Purpose

    FRET-based reporter for Ras activity, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S
    

    Expression

    Mammalian

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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