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pBR_attB(C31)_FRT

Plasmid

#183761

Purpose

phiC31-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-UFM1-dCS

Plasmid

#139869

Purpose

Mammalian expression of HA-tagged human UFM1-cCS

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Mutation

d84-85

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Expression

Bacterial

Available Since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pLenti-X1-Neo-TEX264-HA

Plasmid

#139866

Purpose

Lentiviral expression of HA tagged (C terminal) human TEX264

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

TEX264 (TEX264 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-eKR2

Plasmid

#115337

Purpose

Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

Depositor

Peter Hegemann

Article

Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

Insert

eKR2

Tags

EYFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-ReaChR-P2A-dTomato

Plasmid

#114368

Purpose

expresses a Cre-dependent red light-shifted channelrhodopsin variant

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

ReaChR-P2A-dTomato

Use

AAV

Tags

dTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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FCK-Mac-GFP

Plasmid

#22223

Purpose

Lentiviral expression of Mac-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

NO

Promoter

CaMKII

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pUB-spHRed

Plasmid

#84734

Purpose

Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

pHRed

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Arch-GFP

Plasmid

#37810

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pUB-sSypHer (RbcS-SypHer)

Plasmid

#84732

Purpose

Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

sSypHer

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Nterm_His

Plasmid

#220482

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-HiFi-nCas9n-A72

Plasmid

#125646

Purpose

micro-injection of poly-adenylated HiFi-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

HiFi-nCas9n

Use

Rna in vitro transcription vector

Mutation

R691A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-hu FceRI beta

Plasmid

#8366

Depositor

Jean Kinet

Article

Kuster et al J Biol Chem 1992 Jun 25;267(18):12782-7.

Insert

human Fc epsilon RI beta cDNA (MS4A2 Human)

Expression

Bacterial

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
pLenti-X1-Neo-NDUFB2

Plasmid

#139841

Purpose

Lentiviral expression of human NDUFB2

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB2 (NDUFB2 Human)

Use

Lentiviral

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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iLID-mCherry-iTrkA

Plasmid

#136509

Purpose

Mammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mCherry iTrkA (Ntrk1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Mac-GFP

Plasmid

#58853

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Mac-GFP

Plasmid

#58849

Purpose

AAV expression of Mac-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-NDUFB4

Plasmid

#139840

Purpose

Lentiviral expression of human NDUFB4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB4 (NDUFB4 Human)

Use

Lentiviral

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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