Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 75,733 results for: LAS

Showing: 7981 - 8000 of 75733 results

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Anti-FGF14/FHF4 [N56/21R-2b]

Plasmid

#219479

Purpose

Mammalian Expression Plasmid of anti-FGF14/FHF4 (Mouse) subclass-switched IgG2b R-mAb. Derived from hybridoma N56/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-FGF14/FHF4 (Mus musculus) recombinant mouse monoclonal antibody. (Fgf14 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R-2b]

Plasmid

#220431

Purpose

Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N37A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody. (KCNQ1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUt-mNF-BACH1 Cas9-Resistant

Plasmid

#199218

Purpose

LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

hTetR::P2A::EGFP::BACH1 (BACH1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

silient mutation at nucleic acid 177 C changed to…

Promoter

CMV D2ir promoter

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP

Plasmid

#176283

Purpose

Recombinant rabies virus expressing histone-EGFP

Depositor

Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS2SΔC-H2gp41

Plasmid

#170389

Purpose

Generation of SARS-CoV-2 spike pseudotyped lentivirus

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Tags

HIV gp41

Expression

Mammalian

Mutation

Deletion of C terminal and fusion with H2 from HI…

Promoter

CMV

Available Since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2.75 Spike

Plasmid

#190012

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.2.75 Spike (S )

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CoV2-Spike-D614G-Epsilon

Plasmid

#177964

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

Plasmid

#193359

Purpose

self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-K417N

Plasmid

#177969

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, K417N) (S )

Expression

Mammalian

Mutation

D614G, K417N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N439K

Plasmid

#177970

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N439K) (S )

Expression

Mammalian

Mutation

D614G, N439K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-L452R

Plasmid

#177971

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, L452R) (S )

Expression

Mammalian

Mutation

D614G, L452R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S477N

Plasmid

#177972

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S477N) (S )

Expression

Mammalian

Mutation

D614G, S477N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N501Y

Plasmid

#177975

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (JN.1)

Plasmid

#241117

Purpose

Expresses SARS-CoV-2 spike (JN.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (JN.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

ins16MPLF,T19I,R21T,L24del,P25del,P26del,A27S,S50…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.86)

Plasmid

#241090

Purpose

Expresses SARS-CoV-2 spike (BA.2.86) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.86) (S SARS-CoV-2)

Expression

Mammalian

Mutation

ins16MPLF, T19I, R21T, L24del, P25del, P26del, A2…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2-p2a-KLF4

Plasmid

#193358

Purpose

VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S13I

Plasmid

#177965

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S13I) (S )

Expression

Mammalian

Mutation

D614G, S13I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-T20N

Plasmid

#177966

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, T20N) (S )

Expression

Mammalian

Mutation

D614G, T20N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-P26S

Plasmid

#177967

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P26S) (S )

Expression

Mammalian

Mutation

D614G, P26S

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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