We narrowed to 32,570 results for: Eng

1. pCRISPRyl_D17

   Plasmid

   #84611

   Purpose

   Introduces DSB at D17 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLSLGFP

   Plasmid

   #51494

   Purpose

   pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GFP
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS4083

   Plasmid

   #131739

   Purpose

   Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   sTRSV
   

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRyl_A08

   Plasmid

   #84610

   Purpose

   Introduces DSB at A08 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24b-BG1.3

   Plasmid

   #199437

   Purpose

   expresses BAmGreen1.3 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen1.3
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-iRFP720

   Plasmid

   #89687

   Purpose

   pCAG promoter driving iRFP720

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl_MFE1

   Plasmid

   #84612

   Purpose

   Introduces DSB at MFE1 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS4102

   Plasmid

   #131742

   Purpose

   Xanthine switch Xan-ACGAG

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   Xanthine switch Xan-ACGAG
   

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECO200

   Plasmid

   #169765

   Purpose

   Express SpCas9 and Basta resistance gene

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Plant binary vector

   Expression

   Plant

   Promoter

   35S, AtU6

   Available Since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJPC12-ΔM13-mCherry-PR/PRM-GFP

    Plasmid

    #80859

    Purpose

    Reporter plasmid (mCherry and GFP)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    mCherry
    GFP
    

    Expression

    Bacterial

    Promoter

    Lambda Pr and Lambda Prm

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECO300

    Plasmid

    #169766

    Purpose

    Express SpCas9 and Kanamycin resistance gene

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Plant binary vector

    Expression

    Plant

    Promoter

    35S, AtU6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT_rHCaMP

    Plasmid

    #187107

    Purpose

    Expression of rHCaMP in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    rHCaMP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_EF1a-->GssmCherry-PNE

    Plasmid

    #162157

    Purpose

    Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1025

    Plasmid

    #111424

    Purpose

    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST1374

    Plasmid

    #13426

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLNC Wnt-5bHA

    Plasmid

    #18034

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5b (Wnt5b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pAPInt-Dendra2-Nat

    Plasmid

    #236494

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    Dendra2
    

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETB-roGFP1-R12

    Plasmid

    #83311

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

    Insert

    roGFP1-R12
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

    Promoter

    T7

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pHAGE-TO-MCP-3XBFPnls

    Plasmid

    #75383

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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