We narrowed to 34,025 results for: Spr

1. e-ciCas9_pcDNA5

   Plasmid

   #100553

   Purpose

   Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   e-ciCas9
   

   Use

   CRISPR; Frt, flp-in

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6638

   Plasmid

   #106165

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ915v2+Nterm Spe1 +Cterm Age1 site

   Plasmid

   #88914

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. gRNA-mCtnnb1

   Plasmid

   #84542

   Purpose

   mCtnnb1 gRNA expressing vector

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   mCtnnb1 gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21c-IF3mt

   Plasmid

   #31078

   Depositor

   Linda Spremulli

   Article

   Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

   Insert

   Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Ami…

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pMOD_A0908

   Plasmid

   #91027

   Purpose

   Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2-P2A-AtCas9
   

   Use

   CRISPR

   Promoter

   EC1.2

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin alpha5(1-623)-AHCys

   Plasmid

   #27301

   Depositor

   Timothy Springer

   Article

   Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

   Insert

   integrin alpha 5 (ITGA5 Human)
   

   Expression

   Mammalian

   Mutation

   alpha 5(1-623)+GG+TEV+GGEN+AHCys

   Available Since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pDGE8

   Plasmid

   #79463

   Purpose

   sgRNA shuttle vector; module 2E

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. human b1 ecto-pIRES

   Plasmid

   #113387

   Purpose

   Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

   Insert

   human beta 1 ectodomain
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtCas9_1

    Plasmid

    #91156

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101107

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE12

    Plasmid

    #79469

    Purpose

    sgRNA shuttle vector; module 5

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE13

    Plasmid

    #79471

    Purpose

    sgRNA shuttle vector; module 6

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE14

    Plasmid

    #79473

    Purpose

    sgRNA shuttle vector; module 7

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE15

    Plasmid

    #79474

    Purpose

    sgRNA shuttle vector; module 8E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE10

    Plasmid

    #79466

    Purpose

    sgRNA shuttle vector; module 4

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pfTRAP

    Plasmid

    #50048

    Purpose

    Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    plamodium falciparum TRAP (TRAP Synthetic)
    

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pfTRAP(VWA)

    Plasmid

    #50049

    Purpose

    Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    pfTRAP(vwa) (TRAP Synthetic)
    

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAct:dCas9-scFv

    Plasmid

    #78900

    Purpose

    Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scFV-VP64
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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