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pHH0103_CACNB3-1/1-SV2/2

Plasmid

#91290

Purpose

Protein expression and purification of human SH3 domain construct CACNB3-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB3-1/1-SV2/2 (CACNB3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAPK8IP2-1/1-SV2/2

Plasmid

#91293

Purpose

Protein expression and purification of human SH3 domain construct MAPK8IP2-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAPK8IP2-1/1-SV2/2 (MAPK8IP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO1E-1/1-SV2/2

Plasmid

#91259

Purpose

Protein expression and purification of human SH3 domain construct MYO1E-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO1E-1/1-SV2/2 (MYO1E Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAV2_hSyn_Aurora_CA_Citrine

Plasmid

#98219

Purpose

Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

Depositor

Simon Wiegert

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora_C128A gene

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

Promoter

human synapsin

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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csd6_4-330/pET28b(+) N-tag

Plasmid

#71351

Purpose

csd6, construct:4-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330/pET28b(+) N-tag

Plasmid

#71352

Purpose

csd6, construct:13-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_1-330/pET28b(+) N-tag

Plasmid

#71350

Purpose

csd6, construct:1-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
Ad:2CMV_A2R2-N25K3

Plasmid

#122242

Purpose

Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS2

Plasmid

#185137

Purpose

non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(S345D)-2A-mCherry-puro

Plasmid

#231975

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

MLKL S345D

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(wt)--2A-mCherry-puro

Plasmid

#231974

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS1

Plasmid

#185136

Purpose

non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A3-Neo

Plasmid

#189744

Purpose

The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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