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pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJT103

Plasmid

#25087

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

PompC-lacZ

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-hphMX4

Plasmid

#26876

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pAG505-H2B-frFAST

Plasmid

#160056

Purpose

Expresses H2B-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

H2B-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pLlac01

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Synthetic, Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_W

Plasmid

#173784

Purpose

The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Wphi Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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