We narrowed to 10,634 results for: SEC
-
Plasmid#31901DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only
-
pAcUW51-CgE
Plasmid#13762DepositorInsertHSV-1 glycoprotein E
TagsHisExpressionInsectMutationC-terminal region of the HSV-1 gE (KOS strain ect…Available SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Lckg2a,Y394F)
Plasmid#177893PurposeBaculo expression of His10 tag fused to human LckG2ADepositorAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(L)Cas9
Plasmid#127385PurposeExpresses Cas9 at low levelDepositorInsertuORF(L)-Cas9
UseCRISPRExpressionInsectAvailabilityAcademic Institutions and Nonprofits only -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorMutationsee comments section belowAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster vasaAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MbPylRS(DiZPK)
Plasmid#91706Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interestDepositorInsertspylS
pylT
ExpressionMammalianMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257AAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-S
Plasmid#172426PurposeExpression of full-length, human GIRK2 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPIC9K_A2AR_316_V229C_R291A
Plasmid#246917PurposeExpress A2AR mutants in Pichia PastorisDepositorInsertAdenosine A2A receptor (ADORA2A Human)
Tags10xHis and alpha-factor secretion signal, FLAGExpressionYeastMutationtruncated to aa 1-316, V229C, R291APromoterAOX1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralExpressionInsectPromoterCMV and U6 and H1Available SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL3617 (pCRISPR(BbsI)_Pse)
Plasmid#200902PurposeExpresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.DepositorInsertPseCRISPR (entry)
ExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
Plasmid#133560PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locusDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV5B+longBRD4
Plasmid#157792PurposeExpression of long isoform of BRD4DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only