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pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV OMM-FKBP-V5-sTurboID (N)

Plasmid

#153006

Purpose

Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

OMM (Tom20)-sTurboID (N) (L73)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tTA

Plasmid

#99118

Purpose

An AAV genome that ubiquitously expresses tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV2

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + codon optimized Brn3a

Plasmid

#86829

Purpose

mammalian expression of the transcription factor Brn3a

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

Brn3a (POU4F1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hMLKL

Plasmid

#208789

Purpose

Inducibly expresses human MLKL, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MLKL (MLKL Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pREDTKI

Plasmid

#51628

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum

Plasmid

#20030

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAL119-TK

Plasmid

#21911

Depositor

Maria Castro

Article

Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pGWB401

Plasmid

#74795

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-hCA4-miR122

Plasmid

#244908

Purpose

AAV transgene plasmid encoding human CA4 with miR-122 target site

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

human CA-IV

Use

AAV

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
hUNG1 Y147A
Neo

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Cdk2-HA

Plasmid

#1884

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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GST-p25

Plasmid

#24896

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p25 (CDK5R1 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal truncated version of p35, contains aa …

Available Since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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