We narrowed to 14,429 results for: nts

1. pLV.U6.gDNMT1.Cas9-T2A-GFP

   Plasmid

   #170362

   Purpose

   Plasmid used for Crispr/cas9 based disruption of human DNMT1.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV_EF1a_CDK4-P2A-Hygro_Barcode

   Plasmid

   #170223

   Purpose

   Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1053 pBabe puroL Akt K179M T308A S473A

   Plasmid

   #9013

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-LYRa11-RBD-8h

   Plasmid

   #161822

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T324-D522)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR2.1_HBB_N

   Plasmid

   #87847

   Purpose

   pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

   Depositor

   Marina Kleanthous

   Article

   Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

   Insert

   Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUPI hT5-S322-Cyp

   Plasmid

   #80282

   Purpose

   Expresses human TRIM5 CypA fusion

   Depositor

   Jeremy Luban

   Article

   Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

   Insert

   TRIM5 CypA fusion (TRIM5, PPIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Venus-MDGA2

   Plasmid

   #82544

   Purpose

   Overexpression vector for Venus-MDGA2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA2 (Mdga2 Rat)
   

   Use

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta actin

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-SARS-1-RBD-8h

   Plasmid

   #161821

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC/CMV-JAK1K908E-VSV

   Plasmid

   #139357

   Purpose

   Expression of kinase-dead JAK1-K908E protein

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   K908E

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLS2-YFPc

    Plasmid

    #87166

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiPGK Puro DEST JNKKTR EE Clover

    Plasmid

    #90239

    Purpose

    Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (EE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLS2-YFPn

    Plasmid

    #87165

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    YFPn

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriex-mVenus-PI-RhoA(Q63L)

    Plasmid

    #86978

    Purpose

    PI(DM): Photo-activatable/light sensitive

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    RhoA (RHOA Human)
    

    Use

    Tags

    6xHis and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 LIC 6A FLAG-TEV-DPP8

    Plasmid

    #166795

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD 44 pGL4basic

    Plasmid

    #78135

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    CD 44 (CD44 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CD44

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Rs7327-RBD-8h

    Plasmid

    #161823

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac HTB DPP9L

    Plasmid

    #166788

    Purpose

    Insect cell expression vector for DPP9L (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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