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Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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5HT2C-RNA1-FLAG

Plasmid

#79678

Purpose

serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

5HT2C-RNA1 (HTR2C Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
peGFP-HDAC1 (pc2447)

Plasmid

#240148

Purpose

Expression vector containing a fusion protein of eGFP and human HDAC1.

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

Insert

HDAC1 (HDAC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-diLACCO1

Plasmid

#208102

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-MAT2B

Plasmid

#34795

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

methionine adenosyltransferase II, beta (MAT2B Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-LOC57019

Plasmid

#34811

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced apoptosis inhibitor 1 (CIAPIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pEasyG2_hph

Plasmid

#184915

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mEGFP

Plasmid

#196870

Purpose

Expression of the gamma-tubulin fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mEGFP (Tubg1 Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1

Plasmid

#160051

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pF149 pSOD1G85RAcGFP1

Plasmid

#26410

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G85R

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L861Q)

Plasmid

#32068

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L861Q

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pET21a Wb-iPGM

Plasmid

#180246

Purpose

Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Wuchereria bancrofti iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform2

Plasmid

#70174

Purpose

Mammalian expression of hsOPA1 isoform 2

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 2 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD2

Plasmid

#21401

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN1 (EGLN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pF147 pSOD1A4VAcGFP1

Plasmid

#26408

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only