We narrowed to 21,350 results for: Cre

Showing: 8101 - 8120 of 21350 results

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human-GFAP-EGFP

Plasmid

#169473

Purpose

We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBK-VRER-BE3

Plasmid

#85173

Purpose

Expresses VRER-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VRER-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Puro-iNgn3

Plasmid

#75337

Purpose

doxycycline inducible Ngn3 over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

neurogenin 3 (Neurog3 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Article

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pmiHiFiCas9

Plasmid

#164832

Purpose

miHiFiCas9 expressing plasmid

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miHiFiCas9 (contains Brex27 at sequence 5031-5138).

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

change R 691 to A

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aalb_743

Plasmid

#176676

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LentiAsCpf1-3xMYC-blast

Plasmid

#127750

Purpose

Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

Depositor

Giulio Draetta

Article

Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

Insert

AsCpf1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-SEC62

Plasmid

#139865

Purpose

Lentiviral expression of HA-tagged human SEC62

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

SEC62 (SEC62 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK1010 (pSL25)

Plasmid

#191005

Purpose

Expresses blue chromoprotein on a broad host range RP4 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

blue chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB424

Plasmid

#74818

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Article

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Tags

V5

Expression

Mammalian

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM3Dq-mCherry

Plasmid

#161575

Purpose

Expression of hM3Dq-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM3Dq-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-ERK-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb-DN

Plasmid

#109580

Purpose

Multisite gateway vector for 3' tagging with dominant negative RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Dynamitin-GFP

Plasmid

#51419

Purpose

expression of GFP-tagged wild-type dynamitin p50 in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

Dynamitin (DCTN2 Chicken)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-CIBN-BLITz1-TetR-VP16

Plasmid

#89878

Purpose

expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

TM-CIBN-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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