We narrowed to 17,731 results for: SHE

1. pFRT-TODestRFP_HuD

   Plasmid

   #65766

   Purpose

   expresses RFP-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V23M mutation in RFP

   Promoter

   CMV, 2x Tet

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBbB8c-GFP

   Plasmid

   #35362

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hACE2-cMYC-Flag

   Plasmid

   #158957

   Purpose

   Used for expression of human ACE2 with AAV packaging

   Depositor

   Akiko Iwasaki

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   AAV

   Tags

   Flag and MYC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TODestGFP_HuD

   Plasmid

   #65763

   Purpose

   expresses GFP-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GluA1-scFV-Halo

   Plasmid

   #230065

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

   Plasmid

   #225433

   Purpose

   Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

   Depositor

   Diego Orzaez

   Insert

   4xCBS:mDFR:Rep/RepA:Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPX3

   Plasmid

   #38899

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GPX3 (GPX3 Human)
   

   Use

   Tags

   Tev His Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pKA-142

   Plasmid

   #79835

   Purpose

   Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   PpsR2-mVenus-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMVd1

   Available since

   Aug. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. RHPMP132 (mEOS3.1-beta Actin)

    Plasmid

    #88836

    Purpose

    Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1

    Depositor

    Ricardo Henriques

    Insert

    beta-Actin (ACTB Human)
    

    Use

    Tags

    mEOS3.1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNC-Green-LUC

    Plasmid

    #193438

    Purpose

    Nimble cloning-compatible vector for the analysis of promoter activity in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid

    #154398

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SARS-CoV2 N Protein (N SARS-CoV2)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-AsCas12f1-v5.1+T1-empty

    Plasmid

    #204977

    Purpose

    AsCas12f1-v5.1+T1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nature Catalysis (2023) 6, 695–709

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N70Q/K103R/A104R/S118A/D364R

    Promoter

    PCMV,U6

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbE5a-RFP

    Plasmid

    #35277

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS2a-RFP

    Plasmid

    #35328

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbB6k-GFP

    Plasmid

    #35357

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. RHPMP133 (mOrange2-beta Actin)

    Plasmid

    #88837

    Purpose

    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2

    Depositor

    Ricardo Henriques

    Insert

    beta-Actin (ACTB Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AURKB

    Plasmid

    #39119

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pDONR221-APN

    Plasmid

    #158440

    Purpose

    Gateway Cloning compatible entry vector for the human APN gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid

    #80077

    Purpose

    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

    Depositor

    Phillip Abbosh

    Inserts

    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    

    Use

    Retroviral

    Tags

    5' FLAG

    Expression

    Mammalian

    Mutation

    Y641F, R342R, N393N

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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