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pcDNA3.1-LSSmGFP

Plasmid

#186543

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB660-CYCD3;1

Plasmid

#140751

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Tags

TagRFP

Expression

Plant

Promoter

CaMV 35 promoter

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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ClopHensorN

Plasmid

#50758

Purpose

Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

Depositor

Colin Akerman

Article

Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Insert

ClopHensorN

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Promoter

CAG

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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eMagA-EGFP-Mito

Plasmid

#162244

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-EGFP-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Tags

mEos2

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-mhYFP

Plasmid

#186532

Purpose

Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-S147P/L195M/V206K

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL70004

Plasmid

#50334

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-sspB-Sec61

Plasmid

#247297

Purpose

Mammalian expression of mCherry-sspB-Sec61 fusion; for optogenetic control of protein recruitment to the ER

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

mCherry-sspB-Sec61

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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