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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2930

Plasmid

#175283

Purpose

Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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PROM_MpUBE2

Plasmid

#136109

Purpose

PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEPQD1CB0817

Plasmid

#177039

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoter

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa

Use

Synthetic Biology

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmyo-3

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRSAN-QUEEN 84BP

Plasmid

#129378

Purpose

a variation of QUEEN ATP sensor

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN TogBP (84BP) N/A

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-QUE37C/pLBS CAG

Plasmid

#129322

Purpose

ATP measurement, nucleus, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ZnRed

Plasmid

#170782

Purpose

cytosolic ZnRed zinc sensor with 166 nM and 20 uM Kd

Depositor

Huiwang Ai

Publication

Chen et al Anal Chem. 2016 Sep 20;88(18):9029-36. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ZnRed

Expression

Mammalian

Mutation

K255N

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SNX10-PX (1-201)

Plasmid

#119091

Purpose

Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

Depositor

Brett Collins

Publication

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX10-PX (1-201) (SNX10 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG031

Plasmid

#113994

Purpose

Level 0 module. IPTG promoter for inducible expression

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

IPTG-inducible promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOGG015

Plasmid

#113989

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 5

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOGG013

Plasmid

#113987

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 3

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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yCp50-PRP2

Plasmid

#111304

Purpose

Prp2 in yCp50 plasmid

Depositor

Christine Guthrie

Publication

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP2

Expression

Yeast

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRS313-Sad1-Wt

Plasmid

#90308

Purpose

yeast expression

Depositor

Christine Guthrie

Publication

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

no tag

Expression

Yeast

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-CFP-HA no-pA

Plasmid

#82591

Purpose

3' entry vector containing HA epitope fused to c-terminus of CFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECFP-HA (no pA)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS269

Plasmid

#73727

Purpose

SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Felicitas Proels, Martin Scaal

Publication

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only