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We narrowed to 10,561 results for: fus

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Showing: 8161 - 8180 of 10561 results

  2. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  5. NK-1 GFP

    Plasmid
    #24508
    Depositor
    Ron Vale
    Article
    Case et al Cell. 1997 Sep 5. 90(5):959-66.
    Insert
    Drosophila NCD, human kinesin
    Tags
    GFP and His
    Expression
    Bacterial
    Mutation
    NK-1 is a chimera where the entire superfamily-co…
    Available Since
    June 30, 2010
    Availability
    Academic Institutions and Nonprofits only

  6. LentiGuide Cyclone-Puro P2A EGFP

    Plasmid
    #247497
    Purpose
    Acyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion protein
    Depositor
    Samie Jaffrey
    Article
    Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4
    Insert
    puromycin n-acetyltransferase with Cyclone insertion
    Use
    Lentiviral and Synthetic Biology
    Tags
    Puro(Cyclone)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Cyclone was inserted after Glutamine135 of puromy…
    Promoter
    EF-1a
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    247497-plasmid-map-sequence-id-493033

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  9. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid
    #168099
    Purpose
    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nUbc-OGA(544-706)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    168099-plasmid-map-sequence-id-336059

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  10. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  11. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  12. cryS96

    Plasmid
    #67519
    Purpose
    αB-crystallin minipeptide fused to ELP (S96).
    Depositor
    Andrew MacKay
    Article
    Wang et al J Control Release. 2014 Oct 10;191:4-14.
    Insert
    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
    Tags
    elastin-like polypeptide (ELP) S96
    Expression
    Bacterial
    Mutation
    mini-αB-crystallin contains residues 73–92 of aB-…
    Promoter
    T7
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

    Plasmid
    #181851
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lysine 128 in the DnaJB1-PKAcat fusion changed to…
    Promoter
    CMV
    Available Since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181851-plasmid-map-sequence-id-364311

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  14. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid
    #113961
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113961-plasmid-map-sequence-id-215291

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  15. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    Pfus1mut
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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Showing: 8161 - 8180 of 10561 results