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pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Tags

MGHHHHHHGGA

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CaM-V5-sTb(N)

Plasmid

#219779

Purpose

sTb(N) half of CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pIOM15

Plasmid

#20869

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aadA

Expression

Bacterial

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pBLO1811_arC9_noNLS_human

Plasmid

#74494

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Use

CRISPR

Tags

t2a mCherry (C-terminal on insert)

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8 eArchT3.0-EYFP

Plasmid

#89538

Purpose

Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

Insert

eArchT 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

PRSx8

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+12)

Plasmid

#199161

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+12)

Tags

MGHHHHHHGGA

Mutation

E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-pchG WT

Plasmid

#87107

Purpose

expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

Insert

pchG WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Paxillin-22

Plasmid

#58024

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGST2-TSG101 (2-145)

Plasmid

#184810

Purpose

Bacterial expression of TSG101 UEV domain (aa 2-145)

Depositor

James Hurley

Article

Im et al Structure. 2010 Nov 10. 18(11):1536-47.

Insert

TSG101 (TSG101 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-145 only

Promoter

tac

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAV0532

Plasmid

#133502

Purpose

Expression of mTagBFP2 targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v1

Plasmid

#97081

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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BASIC_8_RBS34- mCherry -B0015

Plasmid

#68142

Purpose

storage vector for BASIC part: RBS-RFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS34-mcherry-B0015

Expression

Bacterial

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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FLS-PC1

Plasmid

#41520

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Polycystin-1 (PKD1 Human)

Tags

Myc/His

Expression

Mammalian

Mutation

sequence from 4107 - 4303 of PKD1

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2_163

Plasmid

#199095

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(Q120L)

Plasmid

#99720

Purpose

Mammalian expression of HA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Mutation

Q120L

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p5954 pcDNA-Xpress-His-USP15 C269A

Plasmid

#23217

Depositor

Peter Howley

Article

Vos et al J Virol. 2009 Sep . 83(17):8885-92.

Insert

USP15 (USP15 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

C269A

Available Since

April 7, 2010

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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